The Genetic Atlas DNA

THE POLYMORPHISM OF NEW Y-STR A10 AND C4 LOCI IN CHINESE

Zheng et al. 2004

Abstract
We have developed a multiplex PCR assay dealing with simultaneously amplifying 9 STR loci on Y-chromosome. These Y-STR markers included DYS434, Y-GATA-A10, DYS438, DYS439, DYS531, DYS557, DYS448, DYS456 and DYS444. A total of 101 different haplotypes was found among 120 unrelated males in a Chinese Han population by using the Y-STR-9-plex system, 91 of them being unique. Gene diversity ranged from 0.4394 at DYS434 to 0.7975 at DYS557. The haplotype diversity value calculated from all nine loci combined was 0.9968. The minimum amount of input DNA that could be used to obtain a full 9 Y-STR profile was 0.5 ng. For the male–male mixtures, the minor component in the mixture could be identified to a ratio of 1:9. In male–female DNA mixtures, the Y-STR-9-plex proved to be highly specific for the Y-chromosome in that no significant female DNA products were observed up to 300 ng of female DNA.